Workshop 1: Biological Applications of X-ray Microscopy and Imaging
Hosted
by Experimental Facilities Division, Argonne National
Laboratory, and
Department of Radiology, Northwestern University Medical
Center
Co-organizers:
Barry Lai, Gayle Woloschak, Jörg Maser, Stefan Vogt,
and Tatjana Paunesku
Bldg. 402, APS Conference Center Lecture Hall (Monday
session)
Bldg. 401, A5000 (Tuesday sessions)
Themes:
Advances in x-ray sources and optics have allowed novel study of biomaterials including cells and microorganisms at sub-optical resolution. Using fluorescent, spectrometric, and imaging approaches, the elemental and chemical state of trace metals, as well as of major constituents, can be studied quantitatively at high sensitivity and with minimum specimen preparation. This workshop, hosted jointly by the APS and the Northwestern University Medical Center, is the second to discuss current capabilities of x-ray microscopy and to identify novel applications. Key contemporary microscopy and imaging techniques will be highlighted, followed by x-ray applications in microbiology, cellular differentiation, and cancer research. Ample time will be allowed for open discussion with feedback from attendees.
| 1:00 p.m. | Registration |
| 1:30 | Welcome/Introduction |
| 1:45 | Quantitative
Determination of Biological Structure and
Composition by Electron Microscopy Richard Leapman, National Institutes of Health |
| 2:15 | Trends
in Optical Microscopy: Current Methodologies and
Novel Approaches to Bioimaging Gary Holtom, Pacific Northwest National Laboratory |
| 2:45 | Applications
of X-ray Micro-fluorescence in Biology and
Jean Susini, European Synchrotron Radiation Facility |
| 3:15 | Refreshments |
| 3:45 | Understanding
the Intracellular Environment of Pathogens: The
Mycobacterial Example Luiz Bermudez, Oregon State University |
| 4:15 | Two
Modalities are Better than One: MicroCT and
Microbeam Diffraction Studies of
Biomineralization Stuart Stock, Northwestern University |
| 4:45 | Diffraction
Enhanced Imaging: New Methods, New
Applications Dean Chapman, Illinois Institute of Technology |
| 6:00 | No-host Dinner, Argonne Guest House |
Tuesday, April 29, 2003; Morning Session
| 9:00 a.m. | Present
Status and Future Development of a Hard X-ray
Microprobe Barry Lai, APS Experimental Facilities Division, Argonne National Laboratory |
| 9:30 | Opening
the Black Box: Trace Element Concentrations and
Distributions in Free-living Marine Protists
Determined with a High Spatial Resolution X-ray
Fluorescence Microprobe Stephen Baines, State University of New York-Stony Brook |
| 10:00 | Hard
X-ray Microprobe Investigations of the
Distributions of Metals in Prokaryotes Ken Kemner, Environmental Research Division, Argonne National Laboratory |
| 10:30 | Refreshments |
| 11:00 | Complementary
Elements: Soft X-ray Spectromicroscopy for Light
Element Chemical Mapping and Analysis Methods Chris Jacobsen, State University of New York-Stony Brook |
| 11:30 | X-Ray
Microprobe Studies of Hemochromatosis Liver Tissues
and Related Microspectroscopy and Spectromicroscopy
Applications T. K. Sham, University of Western Ontario |
| 12:00 | Lunch, Lower-level patio, under the tent |
Tuesday, April 29, 2003; Afternoon Session
| 1:00 p.m. | Clinical
Applications of Microprobe Analysis Ann LeFurgey, Duke University Medical Center |
| 1:30 | The
Chemistry of Biological Molecular Imaging Tom Meade, Northwestern University |
| 2:00 | Control
and Detection of Intracellular Transition Metals:
New Fluorescent Probes for Zinc Cell Biology Tom O'Halloran, Northwestern University |
| 2:30 | Quantitative
and Spatial Changes in Metal Distributions are
Intrinsic and Obligatory Characteristics of Myeloid
Cell Differentiation Dave Glesne, Biochip Technology Center, Argonne National Laboratory |
| 3:00 | Refreshments |
| 3:30 | Probing
the Fate of Platinum (IV) Anticancer Drugs in
Cancer Cells and in Solid Tumours Trevor Hambley, University of Sydney |
| 4:00 | Data
Analysis for X-ray Fluorescence Imaging and its
Application to the Mapping of TiO2-ligonucleotide
Nanocomposites in Mammalian Cells Stefan Vogt, APS Experimental Facilities Division, Argonne National Laboratory |
| 4:30 | Discussion/Conference
Summary Peter Ingram, Discussion Leader, Duke University Medical Center and APS Scientific Advisory Committee |